Ana Sirghi-Marin
PFD Report
Partially Responded
Ref: 2017-0005
Coroner's Concerns (AI summary)
A guideline is needed for immediate microbiological analysis of discolored, non-purulent/non-blood-stained amniotic fluid samples. This precaution is vital for early infection detection, even if not immediately impactful.
View full coroner's concerns
The amniotic fluid drawn off at amniocentesis two days before death was neither purulent nor blood stained, but was discoloured dark yellow.
Given the rarity of such non blood stained discolouration, I heard evidence that it would be a wise precaution in this situation always to send a sample for immediate microbiological analysis, and quickly to follow up the result.
I say always because, at the time of the amniocentesis, there was no fever or other indicator of infection, yet when Ms Sirghi-Marin presented at the emergency unit the following afternoon she was very unwell, and she died the next morning.
Such action would not have changed the outcome in this instance, because presentation to the emergency unit took place approximately 26 hours after the amniocentesis. However, it might in another case. And given the rarity of such non blood stained discoloured amniotic fluid, a guideline that this action is necessary does not seem onerous.
Given the rarity of such non blood stained discolouration, I heard evidence that it would be a wise precaution in this situation always to send a sample for immediate microbiological analysis, and quickly to follow up the result.
I say always because, at the time of the amniocentesis, there was no fever or other indicator of infection, yet when Ms Sirghi-Marin presented at the emergency unit the following afternoon she was very unwell, and she died the next morning.
Such action would not have changed the outcome in this instance, because presentation to the emergency unit took place approximately 26 hours after the amniocentesis. However, it might in another case. And given the rarity of such non blood stained discoloured amniotic fluid, a guideline that this action is necessary does not seem onerous.
Responses
Action Planned
The RCOG will consider the coroner's recommendations regarding bacteriological examination and antibiotic treatment of discoloured amniotic fluid when revising their Green-top guideline. They will also consider adding a prominent notice to their website encouraging doctors to consider these actions. (AI summary)
The RCOG will consider the coroner's recommendations regarding bacteriological examination and antibiotic treatment of discoloured amniotic fluid when revising their Green-top guideline. They will also consider adding a prominent notice to their website encouraging doctors to consider these actions. (AI summary)
View full response
Dear Coroner Hassell,
Re: Prevention of Future Deaths following Amniocentesis
Thank you for your letter dated 9 January 2016. In this you describe the death of a lady, Ana Geanina Sirghi-Marin, 2 days after an amniocentesis, during which the liquor was noted to be discoloured dark yellow but no action taken in response to this unusual appearance. The liquor was not purulent nor blood stained. You have recorded a medical cause of death of:
1a E-coli sepsis 1b Chorioamnionitis 1c Second Trimester Pregnancy
Subsequently, E-coli was grown from the amniotic fluid, indicating that the infection predated the amniocentesis.
You have suggested a wise precaution in future cases – namely to send samples of non-blood stained discoloured amniotic fluid off for culture and quickly to follow-up the result. Although this may not have prevented the death in this case, in other cases it may do so.
We note that you have not suggested that antibiotics might also have been administered in this case. Having read your summary, we think that this should also have been a consideration. Again, whilst it may not have prevented the maternal death, it might do so in future cases.
Current guidelines on amniocentesis were published in 20101 and are due for a renewal. The risk of severe sepsis, including maternal death, is described with a risk < 1/1000 procedures quoted. Whilst emphasis is given to reducing skin contaminants or organisms present on the ultrasound probe and gel by decontamination of probes between patients, and following standard practice to avoid inadvertent puncture of the bowel, no discussion is made on the issue of the finding of discoloured or, for that matter, purulent fluid at amniocentesis. Similarly, the guideline does not mention whether prophylactic antibiotics are justified in all, or in special circumstances and if not, why not.
We have discussed your recommendations with the joint chair of the Royal College of Obstetricians & Gynaecologists’ Guidelines Committee.
They have informed us that the Coroner’s recommendations will be taken into consideration when the next revision of the Green-top guideline is issued, but in order to bring this matter to the attention of specialists performing amniocentesis more quickly, we would recommend that the Royal College of Obstetricians and Gynaecologists consider adding a prominent notice on their website (in particular on the page featuring the guideline for amniocentesis) encouraging doctors to consider sending
amniotic fluid for bacteriological examination as the Coroner has suggested, with consideration of antibiotic treatment if there is evidence of intra-amniotic infection. We suggest the wording of this notice should read:
“If, on inspection of the amniotic fluid following amniocentesis, it has a cloudy or purulent appearance or the patient shows clinical features to suggest intra-amniotic infection, the operator should consider sending a small quantity of amniotic fluid for microbiological analysis and consider antibiotic treatment”.
We hope that the above action is a satisfactory and appropriate response to the findings of your inquest.
Re: Prevention of Future Deaths following Amniocentesis
Thank you for your letter dated 9 January 2016. In this you describe the death of a lady, Ana Geanina Sirghi-Marin, 2 days after an amniocentesis, during which the liquor was noted to be discoloured dark yellow but no action taken in response to this unusual appearance. The liquor was not purulent nor blood stained. You have recorded a medical cause of death of:
1a E-coli sepsis 1b Chorioamnionitis 1c Second Trimester Pregnancy
Subsequently, E-coli was grown from the amniotic fluid, indicating that the infection predated the amniocentesis.
You have suggested a wise precaution in future cases – namely to send samples of non-blood stained discoloured amniotic fluid off for culture and quickly to follow-up the result. Although this may not have prevented the death in this case, in other cases it may do so.
We note that you have not suggested that antibiotics might also have been administered in this case. Having read your summary, we think that this should also have been a consideration. Again, whilst it may not have prevented the maternal death, it might do so in future cases.
Current guidelines on amniocentesis were published in 20101 and are due for a renewal. The risk of severe sepsis, including maternal death, is described with a risk < 1/1000 procedures quoted. Whilst emphasis is given to reducing skin contaminants or organisms present on the ultrasound probe and gel by decontamination of probes between patients, and following standard practice to avoid inadvertent puncture of the bowel, no discussion is made on the issue of the finding of discoloured or, for that matter, purulent fluid at amniocentesis. Similarly, the guideline does not mention whether prophylactic antibiotics are justified in all, or in special circumstances and if not, why not.
We have discussed your recommendations with the joint chair of the Royal College of Obstetricians & Gynaecologists’ Guidelines Committee.
They have informed us that the Coroner’s recommendations will be taken into consideration when the next revision of the Green-top guideline is issued, but in order to bring this matter to the attention of specialists performing amniocentesis more quickly, we would recommend that the Royal College of Obstetricians and Gynaecologists consider adding a prominent notice on their website (in particular on the page featuring the guideline for amniocentesis) encouraging doctors to consider sending
amniotic fluid for bacteriological examination as the Coroner has suggested, with consideration of antibiotic treatment if there is evidence of intra-amniotic infection. We suggest the wording of this notice should read:
“If, on inspection of the amniotic fluid following amniocentesis, it has a cloudy or purulent appearance or the patient shows clinical features to suggest intra-amniotic infection, the operator should consider sending a small quantity of amniotic fluid for microbiological analysis and consider antibiotic treatment”.
We hope that the above action is a satisfactory and appropriate response to the findings of your inquest.
Sent To
- British Maternal and Fetal Medicine Society
- Royal College of Obstetricians and Gynaecologists
Response Status
Linked responses
1 of 2
56-Day Deadline
16 Apr 2017
About PFD responses
Organisations named in PFD reports must respond within 56 days explaining what actions they are taking.
Source: Courts and Tribunals Judiciary
Report Sections
Investigation and Inquest
On 2 August 2016, I commenced an investigation into the death of Ana Geanina Sirghi-Marin, who died on 29 July 2016 aged 36 years. The investigation concluded at the end of the inquest on 5 January 2017. I made a determination at inquest as follows. Ana Sirghi-Marin died because of a naturally occurring uterine infection, when she was sixteen weeks pregnant. She had become pregnant by in vitro fertilisation (IVF) and had undergone an amniocentesis at approximately 1pm on 27 July 2016.
I recorded a medical cause of death of:
1a Escherichia coli sepsis 1b choriamnionitis 1c second trimester pregnancy.
I recorded a medical cause of death of:
1a Escherichia coli sepsis 1b choriamnionitis 1c second trimester pregnancy.
Circumstances of the Death
It appears that the amniotic E coli pre-dated the amniocentesis, as it was later determined to be present in the amniotic fluid drawn off that day.
It is unclear whether conception by IVF played any part in the infective process.
It is unclear whether conception by IVF played any part in the infective process.
Copies Sent To
Care Quality Commission for England
, Chief Medical Officer for England
Director, Homerton University Hospital
Marin
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Data sourced from Courts and Tribunals Judiciary under the Open Government Licence.